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ednapars | 
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% ednapars DNA parsimony algorithm Input (aligned) nucleotide sequence set: dnapars.dat Randomize input order of species [N]: Phylip dnapars program output file [ednapars.outfile]: Create a tree file [Y]: Phylip tree output file [ednapars.treefile]:  | 
Go to the input files for this example
Go to the output files for this example
DNA parsimony algorithm
Version: EMBOSS:6.5.0.0
   Standard (Mandatory) qualifiers (* if not always prompted):
  [-sequence]          seqset     File containing a sequence allignment
*  -random             toggle     [N] Randomize input order of species
*  -randseed           integer    [3] Random number seed (must be odd) (Any
                                  integer value)
*  -randtimes          integer    [3] How many times to randomise (Any integer
                                  value)
*  -outgnum            integer    [1] Number of the outgroup (Integer 1 or
                                  more)
*  -valthresh          float      [1.0] Threshold value (Number 1.000 or more)
  [-outfile]           outfile    [ednapars.outfile] Phylip dnapars program
                                  output file
  [-[no]trout]         toggle     [Y] Create a tree file
*  -treefile           outfile    [ednapars.treefile] Phylip tree output file
   Additional (Optional) qualifiers:
   -[no]besttree       toggle     [Y] Search for best tree
   -og                 toggle     [N] Outgroup root
   -printdata          boolean    [N] Print out the data at start of run
   -progress           boolean    [N] Print indications of progress of run
   -steps              boolean    [N] Print out steps in each site
   -seqatnodes         boolean    [N] Print sequences at all nodes of tree
   -[no]drawtree       boolean    [Y] Draw tree
   Advanced (Unprompted) qualifiers:
   -thresh             toggle     [N] Use Threshold parsimony
   Associated qualifiers:
   "-sequence" associated qualifiers
   -sbegin1            integer    Start of each sequence to be used
   -send1              integer    End of each sequence to be used
   -sreverse1          boolean    Reverse (if DNA)
   -sask1              boolean    Ask for begin/end/reverse
   -snucleotide1       boolean    Sequence is nucleotide
   -sprotein1          boolean    Sequence is protein
   -slower1            boolean    Make lower case
   -supper1            boolean    Make upper case
   -scircular1         boolean    Sequence is circular
   -sformat1           string     Input sequence format
   -iquery1            string     Input query fields or ID list
   -ioffset1           integer    Input start position offset
   -sdbname1           string     Database name
   -sid1               string     Entryname
   -ufo1               string     UFO features
   -fformat1           string     Features format
   -fopenfile1         string     Features file name
   "-outfile" associated qualifiers
   -odirectory2        string     Output directory
   "-treefile" associated qualifiers
   -odirectory         string     Output directory
   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write first file to standard output
   -filter             boolean    Read first file from standard input, write
                                  first file to standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options and exit. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages
   -version            boolean    Report version number and exit
 | 
| Qualifier | Type | Description | Allowed values | Default | 
|---|---|---|---|---|
| Standard (Mandatory) qualifiers | ||||
| [-sequence] (Parameter 1)  | 
seqset | File containing a sequence allignment | Readable set of sequences | Required | 
| -random | toggle | Randomize input order of species | Toggle value Yes/No | No | 
| -randseed | integer | Random number seed (must be odd) | Any integer value | 3 | 
| -randtimes | integer | How many times to randomise | Any integer value | 3 | 
| -outgnum | integer | Number of the outgroup | Integer 1 or more | 1 | 
| -valthresh | float | Threshold value | Number 1.000 or more | 1.0 | 
| [-outfile] (Parameter 2)  | 
outfile | Phylip dnapars program output file | Output file | ednapars.outfile | 
| [-[no]trout] (Parameter 3)  | 
toggle | Create a tree file | Toggle value Yes/No | Yes | 
| -treefile | outfile | Phylip tree output file | Output file | ednapars.treefile | 
| Additional (Optional) qualifiers | ||||
| -[no]besttree | toggle | Search for best tree | Toggle value Yes/No | Yes | 
| -og | toggle | Outgroup root | Toggle value Yes/No | No | 
| -printdata | boolean | Print out the data at start of run | Boolean value Yes/No | No | 
| -progress | boolean | Print indications of progress of run | Boolean value Yes/No | No | 
| -steps | boolean | Print out steps in each site | Boolean value Yes/No | No | 
| -seqatnodes | boolean | Print sequences at all nodes of tree | Boolean value Yes/No | No | 
| -[no]drawtree | boolean | Draw tree | Boolean value Yes/No | Yes | 
| Advanced (Unprompted) qualifiers | ||||
| -thresh | toggle | Use Threshold parsimony | Toggle value Yes/No | No | 
| Associated qualifiers | ||||
| "-sequence" associated seqset qualifiers | ||||
|  -sbegin1 -sbegin_sequence  | 
integer | Start of each sequence to be used | Any integer value | 0 | 
|  -send1 -send_sequence  | 
integer | End of each sequence to be used | Any integer value | 0 | 
|  -sreverse1 -sreverse_sequence  | 
boolean | Reverse (if DNA) | Boolean value Yes/No | N | 
|  -sask1 -sask_sequence  | 
boolean | Ask for begin/end/reverse | Boolean value Yes/No | N | 
|  -snucleotide1 -snucleotide_sequence  | 
boolean | Sequence is nucleotide | Boolean value Yes/No | N | 
|  -sprotein1 -sprotein_sequence  | 
boolean | Sequence is protein | Boolean value Yes/No | N | 
|  -slower1 -slower_sequence  | 
boolean | Make lower case | Boolean value Yes/No | N | 
|  -supper1 -supper_sequence  | 
boolean | Make upper case | Boolean value Yes/No | N | 
|  -scircular1 -scircular_sequence  | 
boolean | Sequence is circular | Boolean value Yes/No | N | 
|  -sformat1 -sformat_sequence  | 
string | Input sequence format | Any string | |
|  -iquery1 -iquery_sequence  | 
string | Input query fields or ID list | Any string | |
|  -ioffset1 -ioffset_sequence  | 
integer | Input start position offset | Any integer value | 0 | 
|  -sdbname1 -sdbname_sequence  | 
string | Database name | Any string | |
|  -sid1 -sid_sequence  | 
string | Entryname | Any string | |
|  -ufo1 -ufo_sequence  | 
string | UFO features | Any string | |
|  -fformat1 -fformat_sequence  | 
string | Features format | Any string | |
|  -fopenfile1 -fopenfile_sequence  | 
string | Features file name | Any string | |
| "-outfile" associated outfile qualifiers | ||||
|  -odirectory2 -odirectory_outfile  | 
string | Output directory | Any string | |
| "-treefile" associated outfile qualifiers | ||||
| -odirectory | string | Output directory | Any string | |
| General qualifiers | ||||
| -auto | boolean | Turn off prompts | Boolean value Yes/No | N | 
| -stdout | boolean | Write first file to standard output | Boolean value Yes/No | N | 
| -filter | boolean | Read first file from standard input, write first file to standard output | Boolean value Yes/No | N | 
| -options | boolean | Prompt for standard and additional values | Boolean value Yes/No | N | 
| -debug | boolean | Write debug output to program.dbg | Boolean value Yes/No | N | 
| -verbose | boolean | Report some/full command line options | Boolean value Yes/No | Y | 
| -help | boolean | Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose | Boolean value Yes/No | N | 
| -warning | boolean | Report warnings | Boolean value Yes/No | Y | 
| -error | boolean | Report errors | Boolean value Yes/No | Y | 
| -fatal | boolean | Report fatal errors | Boolean value Yes/No | Y | 
| -die | boolean | Report dying program messages | Boolean value Yes/No | Y | 
| -version | boolean | Report version number and exit | Boolean value Yes/No | N | 
5 13 Alpha AACGUGGCCAAAU Beta AAGGUCGCCAAAC Gamma CAUUUCGUCACAA Delta GGUAUUUCGGCCU Epsilon GGGAUCUCGGCCC  | 
One most parsimonious tree found:
           +--Epsilon   
        +--4  
     +--3  +--Delta     
     !  !  
  +--2  +-----Gamma     
  !  !  
--1  +--------Beta      
  !  
  +-----------Alpha     
  remember: this is an unrooted tree!
requires a total of     19.000
 | 
((((Epsilon,Delta),Gamma),Beta),Alpha);  | 
| Program name | Description | 
|---|---|
| distmat | Create a distance matrix from a multiple sequence alignment | 
| ednacomp | DNA compatibility algorithm | 
| ednadist | Nucleic acid sequence distance matrix program | 
| ednainvar | Nucleic acid sequence invariants method | 
| ednaml | Phylogenies from nucleic acid maximum likelihood | 
| ednamlk | Phylogenies from nucleic acid maximum likelihood with clock | 
| ednapenny | Penny algorithm for DNA | 
| eprotdist | Protein distance algorithm | 
| eprotpars | Protein parsimony algorithm | 
| erestml | Restriction site maximum likelihood method | 
| eseqboot | Bootstrapped sequences algorithm | 
| fdiscboot | Bootstrapped discrete sites algorithm | 
| fdnacomp | DNA compatibility algorithm | 
| fdnadist | Nucleic acid sequence distance matrix program | 
| fdnainvar | Nucleic acid sequence invariants method | 
| fdnaml | Estimate nucleotide phylogeny by maximum likelihood | 
| fdnamlk | Estimates nucleotide phylogeny by maximum likelihood | 
| fdnamove | Interactive DNA parsimony | 
| fdnapars | DNA parsimony algorithm | 
| fdnapenny | Penny algorithm for DNA | 
| fdolmove | Interactive Dollo or polymorphism parsimony | 
| ffreqboot | Bootstrapped genetic frequencies algorithm | 
| fproml | Protein phylogeny by maximum likelihood | 
| fpromlk | Protein phylogeny by maximum likelihood | 
| fprotdist | Protein distance algorithm | 
| fprotpars | Protein parsimony algorithm | 
| frestboot | Bootstrapped restriction sites algorithm | 
| frestdist | Calculate distance matrix from restriction sites or fragments | 
| frestml | Restriction site maximum likelihood method | 
| fseqboot | Bootstrapped sequences algorithm | 
| fseqbootall | Bootstrapped sequences algorithm | 
This application was modified for inclusion in EMBOSS by Ian Longden (il@sanger.ac.uk) Informatics Division, The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.
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