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helixturnhelix |
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helixturnhelix uses the method of Dodd and Egan to identify helix-turn-helix nucleic acid binding motifs in an input protein sequence. The output is a standard EMBOSS report file describing the location, size and score of any putative motifs.
% helixturnhelix Identify nucleic acid-binding motifs in protein sequences Input protein sequence(s): tsw:laci_ecoli Output report [laci_ecoli.hth]: |
Go to the input files for this example
Go to the output files for this example
Identify nucleic acid-binding motifs in protein sequences
Version: EMBOSS:6.6.0.0
Standard (Mandatory) qualifiers:
[-sequence] seqall Protein sequence(s) filename and optional
format, or reference (input USA)
[-outfile] report [*.helixturnhelix] Output report file name
(default -rformat motif)
Additional (Optional) qualifiers:
-mean float [238.71] Mean value (Number from 1.000 to
10000.000)
-sdvalue float [293.61] Standard Deviation value (Number
from 1.000 to 10000.000)
-minsd float [2.5] Minimum SD (Number from 0.000 to
100.000)
-eightyseven boolean Use the old (1987) weight data
Advanced (Unprompted) qualifiers: (none)
Associated qualifiers:
"-sequence" associated qualifiers
-sbegin1 integer Start of each sequence to be used
-send1 integer End of each sequence to be used
-sreverse1 boolean Reverse (if DNA)
-sask1 boolean Ask for begin/end/reverse
-snucleotide1 boolean Sequence is nucleotide
-sprotein1 boolean Sequence is protein
-slower1 boolean Make lower case
-supper1 boolean Make upper case
-scircular1 boolean Sequence is circular
-squick1 boolean Read id and sequence only
-sformat1 string Input sequence format
-iquery1 string Input query fields or ID list
-ioffset1 integer Input start position offset
-sdbname1 string Database name
-sid1 string Entryname
-ufo1 string UFO features
-fformat1 string Features format
-fopenfile1 string Features file name
"-outfile" associated qualifiers
-rformat2 string Report format
-rname2 string Base file name
-rextension2 string File name extension
-rdirectory2 string Output directory
-raccshow2 boolean Show accession number in the report
-rdesshow2 boolean Show description in the report
-rscoreshow2 boolean Show the score in the report
-rstrandshow2 boolean Show the nucleotide strand in the report
-rusashow2 boolean Show the full USA in the report
-rmaxall2 integer Maximum total hits to report
-rmaxseq2 integer Maximum hits to report for one sequence
General qualifiers:
-auto boolean Turn off prompts
-stdout boolean Write first file to standard output
-filter boolean Read first file from standard input, write
first file to standard output
-options boolean Prompt for standard and additional values
-debug boolean Write debug output to program.dbg
-verbose boolean Report some/full command line options
-help boolean Report command line options and exit. More
information on associated and general
qualifiers can be found with -help -verbose
-warning boolean Report warnings
-error boolean Report errors
-fatal boolean Report fatal errors
-die boolean Report dying program messages
-version boolean Report version number and exit
|
| Qualifier | Type | Description | Allowed values | Default |
|---|---|---|---|---|
| Standard (Mandatory) qualifiers | ||||
| [-sequence] (Parameter 1) |
seqall | Protein sequence(s) filename and optional format, or reference (input USA) | Readable sequence(s) | Required |
| [-outfile] (Parameter 2) |
report | Output report file name | (default -rformat motif) | <*>.helixturnhelix |
| Additional (Optional) qualifiers | ||||
| -mean | float | Mean value | Number from 1.000 to 10000.000 | 238.71 |
| -sdvalue | float | Standard Deviation value | Number from 1.000 to 10000.000 | 293.61 |
| -minsd | float | Minimum SD | Number from 0.000 to 100.000 | 2.5 |
| -eightyseven | boolean | Use the old (1987) weight data | Boolean value Yes/No | No |
| Advanced (Unprompted) qualifiers | ||||
| (none) | ||||
| Associated qualifiers | ||||
| "-sequence" associated seqall qualifiers | ||||
| -sbegin1 -sbegin_sequence |
integer | Start of each sequence to be used | Any integer value | 0 |
| -send1 -send_sequence |
integer | End of each sequence to be used | Any integer value | 0 |
| -sreverse1 -sreverse_sequence |
boolean | Reverse (if DNA) | Boolean value Yes/No | N |
| -sask1 -sask_sequence |
boolean | Ask for begin/end/reverse | Boolean value Yes/No | N |
| -snucleotide1 -snucleotide_sequence |
boolean | Sequence is nucleotide | Boolean value Yes/No | N |
| -sprotein1 -sprotein_sequence |
boolean | Sequence is protein | Boolean value Yes/No | N |
| -slower1 -slower_sequence |
boolean | Make lower case | Boolean value Yes/No | N |
| -supper1 -supper_sequence |
boolean | Make upper case | Boolean value Yes/No | N |
| -scircular1 -scircular_sequence |
boolean | Sequence is circular | Boolean value Yes/No | N |
| -squick1 -squick_sequence |
boolean | Read id and sequence only | Boolean value Yes/No | N |
| -sformat1 -sformat_sequence |
string | Input sequence format | Any string | |
| -iquery1 -iquery_sequence |
string | Input query fields or ID list | Any string | |
| -ioffset1 -ioffset_sequence |
integer | Input start position offset | Any integer value | 0 |
| -sdbname1 -sdbname_sequence |
string | Database name | Any string | |
| -sid1 -sid_sequence |
string | Entryname | Any string | |
| -ufo1 -ufo_sequence |
string | UFO features | Any string | |
| -fformat1 -fformat_sequence |
string | Features format | Any string | |
| -fopenfile1 -fopenfile_sequence |
string | Features file name | Any string | |
| "-outfile" associated report qualifiers | ||||
| -rformat2 -rformat_outfile |
string | Report format | Any string | motif |
| -rname2 -rname_outfile |
string | Base file name | Any string | |
| -rextension2 -rextension_outfile |
string | File name extension | Any string | hth |
| -rdirectory2 -rdirectory_outfile |
string | Output directory | Any string | |
| -raccshow2 -raccshow_outfile |
boolean | Show accession number in the report | Boolean value Yes/No | N |
| -rdesshow2 -rdesshow_outfile |
boolean | Show description in the report | Boolean value Yes/No | N |
| -rscoreshow2 -rscoreshow_outfile |
boolean | Show the score in the report | Boolean value Yes/No | Y |
| -rstrandshow2 -rstrandshow_outfile |
boolean | Show the nucleotide strand in the report | Boolean value Yes/No | Y |
| -rusashow2 -rusashow_outfile |
boolean | Show the full USA in the report | Boolean value Yes/No | N |
| -rmaxall2 -rmaxall_outfile |
integer | Maximum total hits to report | Any integer value | 0 |
| -rmaxseq2 -rmaxseq_outfile |
integer | Maximum hits to report for one sequence | Any integer value | 0 |
| General qualifiers | ||||
| -auto | boolean | Turn off prompts | Boolean value Yes/No | N |
| -stdout | boolean | Write first file to standard output | Boolean value Yes/No | N |
| -filter | boolean | Read first file from standard input, write first file to standard output | Boolean value Yes/No | N |
| -options | boolean | Prompt for standard and additional values | Boolean value Yes/No | N |
| -debug | boolean | Write debug output to program.dbg | Boolean value Yes/No | N |
| -verbose | boolean | Report some/full command line options | Boolean value Yes/No | Y |
| -help | boolean | Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose | Boolean value Yes/No | N |
| -warning | boolean | Report warnings | Boolean value Yes/No | Y |
| -error | boolean | Report errors | Boolean value Yes/No | Y |
| -fatal | boolean | Report fatal errors | Boolean value Yes/No | Y |
| -die | boolean | Report dying program messages | Boolean value Yes/No | Y |
| -version | boolean | Report version number and exit | Boolean value Yes/No | N |
The input is a standard EMBOSS sequence query (also known as a 'USA').
Major sequence database sources defined as standard in EMBOSS installations include srs:embl, srs:uniprot and ensembl
Data can also be read from sequence output in any supported format written by an EMBOSS or third-party application.
The input format can be specified by using the command-line qualifier -sformat xxx, where 'xxx' is replaced by the name of the required format. The available format names are: gff (gff3), gff2, embl (em), genbank (gb, refseq), ddbj, refseqp, pir (nbrf), swissprot (swiss, sw), dasgff and debug.
See: http://emboss.sf.net/docs/themes/SequenceFormats.html for further information on sequence formats.
ID LACI_ECOLI Reviewed; 360 AA.
AC P03023; O09196; P71309; Q2MC79; Q47338;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2003, sequence version 3.
DT 13-JUN-2012, entry version 136.
DE RecName: Full=Lactose operon repressor;
GN Name=lacI; OrderedLocusNames=b0345, JW0336;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=78246991; PubMed=355891; DOI=10.1038/274765a0;
RA Farabaugh P.J.;
RT "Sequence of the lacI gene.";
RL Nature 274:765-769(1978).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Chen J., Matthews K.K.S.M.;
RL Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Marsh S.;
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains
[Part of this file has been deleted for brevity]
FT CHAIN 1 360 Lactose operon repressor.
FT /FTId=PRO_0000107963.
FT DOMAIN 1 58 HTH lacI-type.
FT DNA_BIND 6 25 H-T-H motif.
FT VARIANT 282 282 Y -> D (in T41 mutant).
FT MUTAGEN 17 17 Y->H: Broadening of specificity.
FT MUTAGEN 22 22 R->N: Recognizes an operator variant.
FT CONFLICT 286 286 L -> S (in Ref. 1, 4 and 7).
FT HELIX 6 11
FT TURN 12 14
FT HELIX 17 24
FT HELIX 33 45
FT HELIX 51 56
FT STRAND 63 69
FT HELIX 74 89
FT STRAND 93 98
FT STRAND 101 103
FT HELIX 104 115
FT TURN 116 118
FT STRAND 122 126
FT HELIX 130 139
FT TURN 140 142
FT STRAND 145 150
FT STRAND 154 156
FT STRAND 158 161
FT HELIX 163 177
FT STRAND 181 186
FT HELIX 192 207
FT STRAND 213 217
FT HELIX 222 234
FT STRAND 240 246
FT HELIX 247 259
FT TURN 265 267
FT STRAND 268 271
FT HELIX 277 281
FT STRAND 282 284
FT STRAND 287 290
FT HELIX 293 308
FT STRAND 314 319
FT STRAND 322 324
FT STRAND 334 338
FT HELIX 343 353
FT HELIX 354 356
SQ SEQUENCE 360 AA; 38590 MW; 347A8DEE92D736CB CRC64;
MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ
SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS
GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ
QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT
AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS
VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ
//
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The output is a standard EMBOSS report file.
The results can be output in one of several styles by using the command-line qualifier -rformat xxx, where 'xxx' is replaced by the name of the required format. The available format names are: embl, genbank, gff, pir, swiss, dasgff, debug, listfile, dbmotif, diffseq, draw, restrict, excel, feattable, motif, nametable, regions, seqtable, simple, srs, table, tagseq.
See: http://emboss.sf.net/docs/themes/ReportFormats.html for further information on report formats.
By default helixturnhelix writes a 'motif' report file.
########################################
# Program: helixturnhelix
# Rundate: Mon 15 Jul 2013 12:00:00
# Commandline: helixturnhelix
# -sequence tsw:laci_ecoli
# Report_format: motif
# Report_file: laci_ecoli.hth
########################################
#=======================================
#
# Sequence: LACI_ECOLI from: 1 to: 360
# HitCount: 1
#
# Hits above +2.50 SD (972.73)
#
#=======================================
Maximum_score_at at "*"
(1) Score 2160.000 length 22 at residues 4->25
*
Sequence: VTLYDVAEYAGVSYQTVSRVVN
| |
4 25
Maximum_score_at: 4
Standard_deviations: 6.54
#---------------------------------------
#---------------------------------------
#---------------------------------------
# Total_sequences: 1
# Total_length: 360
# Reported_sequences: 1
# Reported_hitcount: 1
#---------------------------------------
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EMBOSS data files are distributed with the application and stored in the standard EMBOSS data directory, which is defined by the EMBOSS environment variable EMBOSS_DATA.
To see the available EMBOSS data files, run:
% embossdata -showall
To fetch one of the data files (for example 'Exxx.dat') into your current directory for you to inspect or modify, run:
% embossdata -fetch -file Exxx.dat
Users can provide their own data files in their own directories. Project specific files can be put in the current directory, or for tidier directory listings in a subdirectory called ".embossdata". Files for all EMBOSS runs can be put in the user's home directory, or again in a subdirectory called ".embossdata".
The directories are searched in the following order:
The data files are stored in the standard EMBOSS data directory. The names are:
The old (1987) data has a motif length of 20 residues, whilst the default data (Ehth.dat) has a motif length of 22 residues.
With care these can be replaced to suit your data sets. If the files are placed in the following directories they will be used in preference to the files in the EMBOSS distribution data directory:
# Amino acid counts for 91 Helix-turn-helix (presumed) protein motifs # from Dodd IB and Egan JB (1990) Nucl. Acids. Res. 18:5019-5026. # Sample: 91 aligned sequences # # R 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 Total Exp # - -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- ----- --- A 2 1 3 14 10 12 75 6 15 9 1 1 4 3 8 15 4 4 4 11 0 10 212 995 C 0 0 1 1 0 0 0 0 0 3 3 1 1 0 0 0 0 0 0 1 0 3 14 106 D 0 1 0 1 14 0 0 14 1 0 5 0 1 2 0 0 0 0 1 1 0 2 43 556 E 4 5 0 11 26 0 0 16 9 3 3 0 3 12 13 0 0 2 0 1 13 6 127 669 F 4 0 4 0 0 4 0 1 0 10 0 0 0 0 1 0 0 1 1 1 22 0 49 358 G 9 7 1 4 0 0 8 0 0 0 50 0 6 0 7 1 0 3 1 1 0 4 102 761 H 4 3 1 1 2 0 0 3 2 0 5 0 3 3 0 2 0 2 4 5 0 2 42 225 I 10 0 13 3 2 15 0 4 9 4 0 17 0 2 0 1 31 1 4 8 16 1 141 583 K 4 4 6 11 12 1 1 14 11 0 5 2 2 7 2 1 0 5 8 4 5 15 120 516 L 16 1 17 0 1 35 0 3 12 31 0 22 0 2 1 1 22 1 1 12 20 0 198 954 M 7 0 2 1 1 1 0 0 5 7 1 10 0 0 2 0 2 0 0 2 0 1 42 275 N 0 8 0 1 0 0 0 2 1 1 14 0 8 1 4 2 0 4 9 0 0 11 66 383 P 1 6 0 1 0 0 0 0 0 0 0 0 3 13 7 0 0 0 0 0 0 3 34 403 Q 2 1 21 9 11 0 0 9 8 0 0 2 1 17 7 12 0 3 12 5 3 9 132 437 R 9 10 14 9 5 0 1 16 10 0 1 0 1 17 8 7 0 17 28 3 0 16 172 609 S 2 17 0 8 4 1 6 1 2 2 3 0 37 1 25 5 0 29 3 0 1 5 152 552 T 6 24 3 12 1 5 0 2 2 4 0 5 20 4 3 39 0 4 1 0 4 3 142 512 V 7 3 1 1 2 16 0 0 2 12 0 29 0 5 3 3 32 0 7 8 7 0 138 724 W 2 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 2 21 0 0 27 105 Y 2 0 4 3 0 1 0 0 2 4 0 1 1 2 0 2 0 15 5 7 0 0 49 267
The helix-turn-helix protein structural motif was originally identified as the DNA-binding domain of phage repressors. One alpha-helix lies in the wide groove of DNA; the other lies at an angle across DNA. The motif is commonly involved in binding DNA. The motif is of fundamental biological importance and is found in most proteins that regulate gene expression. It is formed by of two alpha-helices joined by a short turn.
| Program name | Description |
|---|---|
| garnier | Predict protein secondary structure using GOR method |
| pepcoil | Predict coiled coil regions in protein sequences |
| pepnet | Draw a helical net for a protein sequence |
| pepwheel | Draw a helical wheel diagram for a protein sequence |
Please report all bugs to the EMBOSS bug team (emboss-bug © emboss.open-bio.org) not to the original author.
Original program "HELIXTURNHELIX" (EGCG 1990) by
Peter Rice
European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK
Please report all bugs to the EMBOSS bug team (emboss-bug © emboss.open-bio.org) not to the original author.