tranalign

 

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Function

Generate an alignment of nucleic coding regions from aligned proteins

Description

tranalign is a re-implementation in EMBOSS of the program mrtrans by Bill Pearson. It reads a set of (unaligned) nucleotide sequences and a corresponding set of aligned protein sequences which are the translations, and writes the coding regions to file as a nucleotide sequence alignment. The sequences must be in the same order in the input sets. Each nucleotide sequence is translated in all three forward frames using the specified genetic code and the translations compared to the corresponding protein sequence from input the alignment. The contiguous nucleotide sequence that coded the protein is written to file (it will not splice together different exons to produce a coding sequence).

Algorithm

The protein sequences will typically include gap (-) characters. These are ignored during sequence comparison but replaced by --- in the nucleotide sequence alignment output.

Usage

Here is a sample session with tranalign 


% tranalign ../data/tranalign.pep tranalign2.seq 
Generate an alignment of nucleic coding regions from aligned proteins

Go to the input files for this example
Go to the output files for this example

Command line arguments 

Generate an alignment of nucleic coding regions from aligned proteins
Version: EMBOSS:6.6.0.0

   Standard (Mandatory) qualifiers:
  [-asequence]         seqall     Nucleotide sequence(s) filename and optional
                                  format, or reference (input USA)
  [-bsequence]         seqset     (Aligned) protein sequence set filename and
                                  optional format, or reference (input USA)
  [-outseq]            seqoutset  [.] (Aligned) nucleotide
                                  sequence set filename and optional format
                                  (output USA)

   Additional (Optional) qualifiers:
   -table              menu       [0] Code to use (Values: 0 (Standard); 1
                                  (Standard (with alternative initiation
                                  codons)); 2 (Vertebrate Mitochondrial); 3
                                  (Yeast Mitochondrial); 4 (Mold, Protozoan,
                                  Coelenterate Mitochondrial and
                                  Mycoplasma/Spiroplasma); 5 (Invertebrate
                                  Mitochondrial); 6 (Ciliate Macronuclear and
                                  Dasycladacean); 9 (Echinoderm
                                  Mitochondrial); 10 (Euplotid Nuclear); 11
                                  (Bacterial); 12 (Alternative Yeast Nuclear);
                                  13 (Ascidian Mitochondrial); 14 (Flatworm
                                  Mitochondrial); 15 (Blepharisma
                                  Macronuclear); 16 (Chlorophycean
                                  Mitochondrial); 21 (Trematode
                                  Mitochondrial); 22 (Scenedesmus obliquus);
                                  23 (Thraustochytrium Mitochondrial))

   Advanced (Unprompted) qualifiers: (none)
   Associated qualifiers:

   "-asequence" associated qualifiers
   -sbegin1            integer    Start of each sequence to be used
   -send1              integer    End of each sequence to be used
   -sreverse1          boolean    Reverse (if DNA)
   -sask1              boolean    Ask for begin/end/reverse
   -snucleotide1       boolean    Sequence is nucleotide
   -sprotein1          boolean    Sequence is protein
   -slower1            boolean    Make lower case
   -supper1            boolean    Make upper case
   -scircular1         boolean    Sequence is circular
   -squick1            boolean    Read id and sequence only
   -sformat1           string     Input sequence format
   -iquery1            string     Input query fields or ID list
   -ioffset1           integer    Input start position offset
   -sdbname1           string     Database name
   -sid1               string     Entryname
   -ufo1               string     UFO features
   -fformat1           string     Features format
   -fopenfile1         string     Features file name

   "-bsequence" associated qualifiers
   -sbegin2            integer    Start of each sequence to be used
   -send2              integer    End of each sequence to be used
   -sreverse2          boolean    Reverse (if DNA)
   -sask2              boolean    Ask for begin/end/reverse
   -snucleotide2       boolean    Sequence is nucleotide
   -sprotein2          boolean    Sequence is protein
   -slower2            boolean    Make lower case
   -supper2            boolean    Make upper case
   -scircular2         boolean    Sequence is circular
   -squick2            boolean    Read id and sequence only
   -sformat2           string     Input sequence format
   -iquery2            string     Input query fields or ID list
   -ioffset2           integer    Input start position offset
   -sdbname2           string     Database name
   -sid2               string     Entryname
   -ufo2               string     UFO features
   -fformat2           string     Features format
   -fopenfile2         string     Features file name

   "-outseq" associated qualifiers
   -osformat3          string     Output seq format
   -osextension3       string     File name extension
   -osname3            string     Base file name
   -osdirectory3       string     Output directory
   -osdbname3          string     Database name to add
   -ossingle3          boolean    Separate file for each entry
   -oufo3              string     UFO features
   -offormat3          string     Features format
   -ofname3            string     Features file name
   -ofdirectory3       string     Output directory

   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write first file to standard output
   -filter             boolean    Read first file from standard input, write
                                  first file to standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options and exit. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages
   -version            boolean    Report version number and exit

Qualifier Type Description Allowed values Default
Standard (Mandatory) qualifiers
[-asequence]
(Parameter 1)		 seqall Nucleotide sequence(s) filename and optional format, or reference (input USA) Readable sequence(s) Required
[-bsequence]
(Parameter 2)		 seqset (Aligned) protein sequence set filename and optional format, or reference (input USA) Readable set of sequences Required
[-outseq]
(Parameter 3)		 seqoutset (Aligned) nucleotide sequence set filename and optional format (output USA) Writeable sequences <*>.format
Additional (Optional) qualifiers
-table list Code to use
0 (Standard)
1 (Standard (with alternative initiation codons))
2 (Vertebrate Mitochondrial)
3 (Yeast Mitochondrial)
4 (Mold, Protozoan, Coelenterate Mitochondrial and Mycoplasma/Spiroplasma)
5 (Invertebrate Mitochondrial)
6 (Ciliate Macronuclear and Dasycladacean)
9 (Echinoderm Mitochondrial)
10 (Euplotid Nuclear)
11 (Bacterial)
12 (Alternative Yeast Nuclear)
13 (Ascidian Mitochondrial)
14 (Flatworm Mitochondrial)
15 (Blepharisma Macronuclear)
16 (Chlorophycean Mitochondrial)
21 (Trematode Mitochondrial)
22 (Scenedesmus obliquus)
23 (Thraustochytrium Mitochondrial)
 0
Advanced (Unprompted) qualifiers
(none)
Associated qualifiers
"-asequence" associated seqall qualifiers
-sbegin1
-sbegin_asequence		 integer Start of each sequence to be used Any integer value 0
-send1
-send_asequence		 integer End of each sequence to be used Any integer value 0
-sreverse1
-sreverse_asequence		 boolean Reverse (if DNA) Boolean value Yes/No N
-sask1
-sask_asequence		 boolean Ask for begin/end/reverse Boolean value Yes/No N
-snucleotide1
-snucleotide_asequence		 boolean Sequence is nucleotide Boolean value Yes/No N
-sprotein1
-sprotein_asequence		 boolean Sequence is protein Boolean value Yes/No N
-slower1
-slower_asequence		 boolean Make lower case Boolean value Yes/No N
-supper1
-supper_asequence		 boolean Make upper case Boolean value Yes/No N
-scircular1
-scircular_asequence		 boolean Sequence is circular Boolean value Yes/No N
-squick1
-squick_asequence		 boolean Read id and sequence only Boolean value Yes/No N
-sformat1
-sformat_asequence		 string Input sequence format Any string  
-iquery1
-iquery_asequence		 string Input query fields or ID list Any string  
-ioffset1
-ioffset_asequence		 integer Input start position offset Any integer value 0
-sdbname1
-sdbname_asequence		 string Database name Any string  
-sid1
-sid_asequence		 string Entryname Any string  
-ufo1
-ufo_asequence		 string UFO features Any string  
-fformat1
-fformat_asequence		 string Features format Any string  
-fopenfile1
-fopenfile_asequence		 string Features file name Any string  
"-bsequence" associated seqset qualifiers
-sbegin2
-sbegin_bsequence		 integer Start of each sequence to be used Any integer value 0
-send2
-send_bsequence		 integer End of each sequence to be used Any integer value 0
-sreverse2
-sreverse_bsequence		 boolean Reverse (if DNA) Boolean value Yes/No N
-sask2
-sask_bsequence		 boolean Ask for begin/end/reverse Boolean value Yes/No N
-snucleotide2
-snucleotide_bsequence		 boolean Sequence is nucleotide Boolean value Yes/No N
-sprotein2
-sprotein_bsequence		 boolean Sequence is protein Boolean value Yes/No N
-slower2
-slower_bsequence		 boolean Make lower case Boolean value Yes/No N
-supper2
-supper_bsequence		 boolean Make upper case Boolean value Yes/No N
-scircular2
-scircular_bsequence		 boolean Sequence is circular Boolean value Yes/No N
-squick2
-squick_bsequence		 boolean Read id and sequence only Boolean value Yes/No N
-sformat2
-sformat_bsequence		 string Input sequence format Any string  
-iquery2
-iquery_bsequence		 string Input query fields or ID list Any string  
-ioffset2
-ioffset_bsequence		 integer Input start position offset Any integer value 0
-sdbname2
-sdbname_bsequence		 string Database name Any string  
-sid2
-sid_bsequence		 string Entryname Any string  
-ufo2
-ufo_bsequence		 string UFO features Any string  
-fformat2
-fformat_bsequence		 string Features format Any string  
-fopenfile2
-fopenfile_bsequence		 string Features file name Any string  
"-outseq" associated seqoutset qualifiers
-osformat3
-osformat_outseq		 string Output seq format Any string  
-osextension3
-osextension_outseq		 string File name extension Any string  
-osname3
-osname_outseq		 string Base file name Any string  
-osdirectory3
-osdirectory_outseq		 string Output directory Any string  
-osdbname3
-osdbname_outseq		 string Database name to add Any string  
-ossingle3
-ossingle_outseq		 boolean Separate file for each entry Boolean value Yes/No N
-oufo3
-oufo_outseq		 string UFO features Any string  
-offormat3
-offormat_outseq		 string Features format Any string  
-ofname3
-ofname_outseq		 string Features file name Any string  
-ofdirectory3
-ofdirectory_outseq		 string Output directory Any string  
General qualifiers
-auto boolean Turn off prompts Boolean value Yes/No N
-stdout boolean Write first file to standard output Boolean value Yes/No N
-filter boolean Read first file from standard input, write first file to standard output Boolean value Yes/No N
-options boolean Prompt for standard and additional values Boolean value Yes/No N
-debug boolean Write debug output to program.dbg Boolean value Yes/No N
-verbose boolean Report some/full command line options Boolean value Yes/No Y
-help boolean Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose Boolean value Yes/No N
-warning boolean Report warnings Boolean value Yes/No Y
-error boolean Report errors Boolean value Yes/No Y
-fatal boolean Report fatal errors Boolean value Yes/No Y
-die boolean Report dying program messages Boolean value Yes/No Y
-version boolean Report version number and exit Boolean value Yes/No N

Input file format

The input is a set of unaligned nucleic sequences and the set of aligned protein sequences to be used as a guide in the alignment of the output nucleic sequences.

The ID names of the nucleic acid and protein sequences are NOT checked to see if they correspond to each other. They can have any names.

There must be at least as many protein sequences as nucleic acid sequence - extra protein sequences are ignored.

Each of the nucleic acid sequences must have a corresponding protein sequence which is derived from the coding region of that nucleic acid sequence. The two sets of sequences must be in the same order.

Input files for usage example

File: tranalign.seq

>HSFAU1
ttcctctttctcgactccatcttcgcggtagctgggaccgccgttcagtcgccaatatgc
agctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacggtcg
cccagatcaaggctcatgtagcctcactggagggcattgccccggaagatcaagtcgtgc
tcctggcaggccccctggaggatgaggccactctgggccagtgcggggtggaggccc
tgactaccctggaagtagcaggccgcatgcttggaggtaaagttcatggttccctggccc
gtgctggaaaagtgagaggtcagactcctaaggtggccaaacaggagaagaagaagaaga
agacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtgccca
cctttggcaagaagaagggccccaatgccaactcttaagtcttttgtaattctggctttc
tctaataaaaaagccacttagttcagtcaaaaaaaaaa
>HSFAU2
ttcctctttctcgactccatcttcgcggtagctgggaccgccgttcagtcgccaatatgc
agctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacggtcg
cccagatcaaggctcatgtagcctcactggagggcattgccccggaagatcaagtcgtgc
tcctggcaggcgcgcccctggaggatgcactctgggccagtgcggggtggaggccc
tgactaccctggaagtagcaggccgcatgcttggaggtaaagttcatggttccctggccc
gtgctggaaaagtgagaggtcagactcctaaggtggccaaacaggagaagaagaagaaga
agacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtgccca
cctttggcaagaagaagggccccaatgccaactcttaagtcttttgtaattctggctttc
tctaataaaaaagccacttagttcagtcaaaaaaaaaa
>HSFAU3
ttcctctttctcgactccatcttcgcggtagctgggaccgccgttcagtcgccaatatgc
agctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacggtcg
cccagatcaaggctcatgtagcctcactggagggcattgccccggaagatcaagtcgtgc
tcctggcaggcgcgcccctggaggatgaggccactctgggccagtgcggggtggaggccc
tgactaccctggaagtagcaggccgcatgcttggaggtaaagttcatggttccctggccc
gtgctggaaaagtgagaggtcagactcctaagggggccaaacaggagaagaagaagaaga
agacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtgccca
cctttggcaagaagaagggccccaatgccaactcttaagtcttttgtaattctggctttc
tctaataaaaaagccacttagttcagtcaaaaaaaaaa
>HSFAU4
ttcctctttctcgactccatcttcgcggtagctgggaccgccgttcagtcgccaatatgc
agctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacggtcg
cccagatcaaggctcatgaaatagcctcactggagggcattgccccggaagatcaagtcgtgc
tcctggcaggcgcgcccctggaggatgaggccactctgggccagtgcggggtggaggccc
tgactaccctggaagtagcaggccgcatgcttgcccgaggtaaagttcatggttccctggccc
gtgctggaaaagtgagaggtcagactcctaaggtggccaaacaggagaagaagaagaaga
agacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtgccca
cctttggcaagaagaagggccccaatgccaactcttaagtcttttgtaattctggctttc
tctaataaaaaagccacttagttcagtcaaaaaaaaaa
>HSFAU5
ttcctctttctcgactccatcttcgcggtagctgggaccgccgttcagtcgccaatatgc
agctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacggtcg
cccagatcaaggctcatgtagcctcactggagggcattgccccggaagatcaagtcgtgc
tcctggcaggcgcgcccctggaggatgaggccactctgggccagtgcggggtggaggccc
tgactaccctggaagtaggccgcatgctttttggaggtaaagttcatggttccctggccc
gtgctggaaaagtgagaggtcagactcctaaggtggccaaacaggagaagaagaagaaga
agacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtgccca
cctttggcaagaagaagggccccaatgccaactcttaagtcttttgtaattctggctttc
tctaataaaaaagccacttagttcagtcaaaaaaaaaa

File: tranalign.pep

>HSFAU1_3
PLSRLHLRGSWDRRSVANMQLFVRAQELHTFEVTGQETVAQIKAHVAS-LEGIAPEDQVV
LLAG-PLEDEATLGQCGVEALTTLEVAGRMLG-GKVHGSLARAGKVRGQTPKVAKQEKKK
KKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS
>HSFAU2_3
PLSRLHLRGSWDRRSVANMQLFVRAQELHTFEVTGQETVAQIKAHVAS-LEGIAPEDQVV
LLAGAPLEDALWASAGWRP
>HSFAU3_3
PLSRLHLRGSWDRRSVANMQLFVRAQELHTFEVTGQETVAQIKAHVAS-LEGIAPEDQVV
LLAGAPLEDEATLGQCGVEALTTLEVAGRMLG-GKVHGSLARAGKVRGQTPKGAKQEKKK
KKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS
>HSFAU4_3
PLSRLHLRGSWDRRSVANMQLFVRAQELHTFEVTGQETVAQIKAHEIASLEGIAPEDQVV
LLAGAPLEDEATLGQCGVEALTTLEVAGRMLARGKVHGSLARAGKVRGQTPKVAKQEKKK
KKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS
>HSFAU5_3
PLSRLHLRGSWDRRSVANMQLFVRAQELHTFEVTGQETVAQIKAHVAS-LEGIAPEDQVV
LLAGAPLEDEATLGQCGVEALTTLEVGRMLFG-GKVHGSLARAGKVRGQTPKVAKQEKKK
KKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS

Output file format

Output files for usage example

File: tranalign2.seq

>HSFAU1
cctctttctcgactccatcttcgcggtagctgggaccgccgttcagtcgccaatatgcag
ctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacggtcgcc
cagatcaaggctcatgtagcctca---ctggagggcattgccccggaagatcaagtcgtg
ctcctggcaggc---cccctggaggatgaggccactctgggccagtgcggggtggaggcc
ctgactaccctggaagtagcaggccgcatgcttgga---ggtaaagttcatggttccctg
gcccgtgctggaaaagtgagaggtcagactcctaaggtggccaaacaggagaagaagaag
aagaagacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtg
cccacctttggcaagaagaagggccccaatgccaactct
>HSFAU2
cctctttctcgactccatcttcgcggtagctgggaccgccgttcagtcgccaatatgcag
ctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacggtcgcc
cagatcaaggctcatgtagcctca---ctggagggcattgccccggaagatcaagtcgtg
ctcctggcaggcgcgcccctggaggatgcactctgggccagtgcggggtggaggccc---
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
---------------------------------------
>HSFAU3
cctctttctcgactccatcttcgcggtagctgggaccgccgttcagtcgccaatatgcag
ctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacggtcgcc
cagatcaaggctcatgtagcctca---ctggagggcattgccccggaagatcaagtcgtg
ctcctggcaggcgcgcccctggaggatgaggccactctgggccagtgcggggtggaggcc
ctgactaccctggaagtagcaggccgcatgcttgga---ggtaaagttcatggttccctg
gcccgtgctggaaaagtgagaggtcagactcctaagggggccaaacaggagaagaagaag
aagaagacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtg
cccacctttggcaagaagaagggccccaatgccaactct
>HSFAU4
cctctttctcgactccatcttcgcggtagctgggaccgccgttcagtcgccaatatgcag
ctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacggtcgcc
cagatcaaggctcatgaaatagcctcactggagggcattgccccggaagatcaagtcgtg
ctcctggcaggcgcgcccctggaggatgaggccactctgggccagtgcggggtggaggcc
ctgactaccctggaagtagcaggccgcatgcttgcccgaggtaaagttcatggttccctg
gcccgtgctggaaaagtgagaggtcagactcctaaggtggccaaacaggagaagaagaag
aagaagacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtg
cccacctttggcaagaagaagggccccaatgccaactct
>HSFAU5
cctctttctcgactccatcttcgcggtagctgggaccgccgttcagtcgccaatatgcag
ctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacggtcgcc
cagatcaaggctcatgtagcctca---ctggagggcattgccccggaagatcaagtcgtg
ctcctggcaggcgcgcccctggaggatgaggccactctgggccagtgcggggtggaggcc
ctgactaccctggaagtaggccgcatgctttttgga---ggtaaagttcatggttccctg
gcccgtgctggaaaagtgagaggtcagactcctaaggtggccaaacaggagaagaagaag
aagaagacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtg
cccacctttggcaagaagaagggccccaatgccaactct

The output is the regions of the nucleic acid sequences which code for the corresponding protein sequence, with gap characters ('-') introduced so that they have the same alignment as the corresponding protein sequences.

Data files

None.

Notes

In general, it is better to use protein sequences for multiple alignment, but to use DNA sequences for phylogeny, for example, when using the programs dnadist, dnapars, dnaml, etc in the PHYLIP package. Where one has a protein sequence alignment, it would be time consuming to remove gap characters before back-translating the proteins. tranalign helps by generating aligned cDNA sequences from a protein sequence alignment.

tranalign finds the coding regions for contiguous sequences only. It will not splice together different exons to produce a coding sequence. You should therefore use either mRNA sequences, or nucleic sequences which you have constructed to hold a contiguous coding region (maybe using extractseq or yank and union?).

References

None.

Warnings

The sequences must be in the same order in both input sets of sequences. Some alignment program (including clustalw/emma) will re-order their input sequences so as to group similar sequences together.

Diagnostic Error Messages

"No guide protein sequence available for nucleic sequence xxx" - the corresponding protein sequence for this nucleic sequence has not been input. You have input more nucleic acid sequences than protein sequences.

"Guide protein sequence xxx not found in nucleic sequence xxx" - the region of the nucleic sequence which codes for the protein was not found. The coding region in the nucleic acid sequence must be a single contiguous sequence. The protein sequence might not be the corresponding one for this nucleic acid sequence if they are out of order.

Exit status

It always exits with status 0.

Known bugs

None.

See also

Program name Description
edialign Local multiple alignment of sequences
emma Multiple sequence alignment (ClustalW wrapper)
infoalign Display basic information about a multiple sequence alignment
plotcon Plot conservation of a sequence alignment
prettyplot Draw a sequence alignment with pretty formatting
showalign Display a multiple sequence alignment in pretty format

Author(s)

The original program mrtrans was written by Bill Pearson (wrp@virginia.edu)

tranalign was written in EMBOSS code using the description of mrtrans as a guide by Gary Williams formerly at:
MRC Rosalind Franklin Centre for Genomics Research Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SB, UK

Please report all bugs to the EMBOSS bug team (emboss-bug © emboss.open-bio.org) not to the original author.

History

mrtrans written (Jan 1991, July 1987) - Bill Pearson

tranalign written (March 2002) - Gary Williams

Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

Comments

None